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Nucleocytoplasmic Ratios in Different Populations of Rat Liver Parenchymal Cells during Azo Dye Carcinogenesis¹

Laboratoires de Recherche, Institut du Cancer de Montréal, Hôpital Notre-Dame et Université de Montréal, Montréal, Canada

Quantitative measurements of nucleocytoplasmic ratios were carried out by Chalkley's method (1) in different populations of preneoplastic liver parenchyma (hypobasophilic, basophilic, and hyperbasophilic cells), as well as in normal and neoplastic tissue. The results showed that an increase in nucleocytoplasmic ratio takes place in hyperbasophilic regions, thus strengthening the view that such regions represent a transition between hyperplasia and neoplasia.

The increased nucleocytoplasmic ratios observed in hyperbasophilic regions and hepatomas are due mainly to an increase in the number of nuclei per unit area. Such change follows other alterations in RNA⁴ staining, DNase activity, DNA synthesis, and mitotic activity associated with the neoplastic transformation.

¹ This investigation was supported by a grant from the National Cancer Institute of Canada to Dr. A. Cantero, Director of the Research Laboratories. A preliminary report of this work was presented at the 55th Annual Meeting of the American Association for Cancer Research held in Chicago, April 9–11, 1964 (4).

⁴ The abbreviations used are: RNA, ribonucleic acid; DNase, deoxyribonuclease; DNA, deoxyribonucleic acid; and DAB, 4-dimethylaminoazobenzene.

² Fellow of the Cancer Research Society.

³ Research Scientist of the National Cancer Institute of Canada.

Received 2/ 3/65.