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Carbohydrate Content in Subcellular Fractions of Rat Liver and of Walker 256 Carcinosarcoma¹

Department of Biochemistry, University of Oklahoma Medical School, Oklahoma City, Oklahoma

The distribution of protein-bound neutral sugars, hexosamine, and sialic acid in the subcellular fractions of the Walker 256 tumor was compared with that in rat liver. Lysosomal, microsomal, and mitochondrial fractions were higher in bound carbohydrate constituents, including hexosamine, sialic acid, mannose, galactose, and fucose, than were the nuclear and soluble fractions. The subcellular fractions of the Walker 256 tumor contained more bound carbohydrates than did those of liver. The molar ratio of sialic acid to hexosamine and the molar ratio of galactosamine to glucosamine were higher in the subcellular fractions of the Walker tumor. Mitochondrial, lysosomal, and microsomal fractions of rat liver contained strikingly greater amounts of mannose than of galactose; this difference was less marked in the subcellular fractions of the Walker tumor.

¹ This investigation was supported by USPHS research grant CA-03081-08 from the National Cancer Institute.

² Currently, Visiting Professor of Biochemistry, University of California Project in Medical Education, Universitas Airlangga Fakultas Kedokteran, Surabaja, Indonesia.

Received 2/17/65.