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Loss of Neoplastic Properties in Vitro

II. Observations on KB Sublines¹

Laboratories of Microbiology and Tumor Transplantation, The Children's Cancer Research Foundation, and Department of Pathology, Harvard Medical School, at The Children's Hospital, Boston, Massachusetts; The Child Research Center of Michigan, Detroit, Michigan (C. S. S.); and The Department of Cell Biology, Albert Einstein Medical College, New York, New York (H. E.)

Ten sublines of the KB cell line obtained from a number of different laboratories were reexamined with respect to chromosome number and heterotransplantability to the Syrian hamster cheek pouch. Twelve clonal cultures derived from 2 of these sublines were similarly examined.

These sublines differed from one another and from the KB cell line originally studied, which was heterotransplantable with inocula of circa 10 cells. Only 2 of the sublines could be heterotransplanted with inocula of less than 10⁴ cells; 6 were heterotransplantable with inocula of 10⁴-10⁶ cells; and 2 failed to heterotransplant, even with inocula of 10⁶ cells. Similar differences in heterotransplantability were observed among the clonal derivatives of 2 of these sublines. Although the modal numbers of these sublines and clonal derivatives were basically similar, ranging from 73 to 80, a few lines had a chromosome of sufficiently distinctive morphology to serve as a marker. There was, however, no apparent correlation between these minor karyotypic differences and heterotransplantability. Possible differences between nonheterotransplantable and heterotransplantable sublines with respect to the pattern of variability in cytoplasmic protein and cytoplasmic ribonucleic acid (RNA) content per cell remain to be studied in detail.

¹ These studies were supported in part by research grants C-6516, CA-02947, and E-4153 from the National Cancer Institute and the National Institute for Allergy and Infectious Diseases, NIH, USPHS, to The Children's Cancer Research Foundation, The Child Research Center of Michigan, and Albert Einstein Medical College, respectively.

² Holds Research Career Award 1-K6-CA-22,150 from the National Cancer Institute.

³ Special Fellow 1-F3-CA-21,216-01, National Cancer Institute; present address: Morristown Memorial Hospital, Morristown, N. J.

Received 12/ 3/64. Revised 4/28/65.