This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Freireich, E. J. Articles by Levin, R. H. Search for Related Content PubMed PubMed Citation Articles by Freireich, E. J. Articles by Levin, R. H.

Separation and Collection of Leukocytes

National Cancer Institute, Bethesda, Maryland, and the International Business Machines Corporation, Endicott, New York

Existing methods for separation and collection of leukocytes permit collection of only limited quantities of leukocytes. An instrument has been designed to process large volumes of blood on a continuous flow basis. The instrument collects venous blood, separates leukocytes in a centrifuge, and reinjects the red cells, plasma, and platelets. The separation process is 30–60% efficient in vitro at flow rates up to 50 ml/min. In vivo tests demonstrated safety, sterility, lack of hemolysis, and adequate anti-coagulation. However, leukocyte recovery in vivo is low (under 20%). If this poor in vivo recovery can be overcome, the instrument should prove useful for collection of large quantities of leukocytes.

¹ Deceased.