This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bryan, G. T. Articles by Springberg, P. D. Search for Related Content PubMed PubMed Citation Articles by Bryan, G. T. Articles by Springberg, P. D.

Role of the Vehicle in the Genesis of Bladder Carcinomas in Mice by the Pellet Implantation Technic¹

Division of Clinical Oncology, University of Wisconsin Medical School, Madison, Wisconsin

Several compounds—arachic acid, hexaethylbenzene, hexamethylbenzene, 1-octadecanol, palmitic acid, and stearamide—were individually compressed as pellets and tested, by implantation into the mouse bladder, as possible vehicles for carcinogenicity experiments. All of these compounds were found to be associated with a 3–17% incidence of bladder carcinomas. Pellets of 20-methylcholanthrene (MC)² used alone induced an incidence of 52%, whereas pellets of XAE used alone induced an incidence of 1.6%. The pellets of XAE disintegrated within 2 days following surgical introduction into the mouse bladder. When cholesterol pellets were placed in the mouse bladder and XAE was injected s.c., a 30% incidence of bladder carcinomas was observed. However, the repeated s.c. injection of XAE into mice whose bladders did not contain cholesterol pellets failed to produce any bladder carcinomas. XAE was observed to be present in the urine of mice following s.c. injection. It was suggested that the prolonged presence of the vehicle in the mouse bladder had a promoting effect on the genesis of the bladder carcinomas. Several metabolites of the essential amino acid tryptophan were quantitatively measured in the urine of mice. It was found that 3-hydroxy-L-kynurenine was present in mouse urine; this had previously been demonstrated to be associated with the production of a significant incidence of mouse bladder carcinomas when implanted in a cholesterol vehicle. It was postulated that the combination of continued excretion of this compound or some other metabolite in the urine and the protracted presence of a pellet resulted in the genesis of a low-background incidence of bladder carcinomas in mice.

¹ Supported in part by Grants E-202C, E-115B, and E-116F from the American Cancer Society; by Grants A-1127 and A-1499 from the National Institutes of Arthritis and Metabolic Diseases; by Grants CA-03274 and Ca-06749 from the National Cancer Institute, USPHS; and by the Elsa U. Pardee Foundation.

² The abbreviations used are: MC, 20-methylcholanthrene, and XAE, 8-methyl ether of xanthurenic acid.

Received 7/ 9/65.

This article has been cited by other articles:

				G. T. Bryan, E. Erturk, and O. Yoshida Production of Urinary Bladder Carcinomas in Mice by Sodium Saccharin Science, June 5, 1970; 168(3936): 1238 - 1240. [Abstract] [PDF]

				G. T. Bryan and E. Erturk Production of Mouse Urinary Bladder Carcinomas by Sodium Cyclamate Science, February 13, 1970; 167(3920): 996 - 998. [Abstract] [PDF]