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An Antigenic Fraction of the Lymphosarcoma 6C3HED of the C3H Mouse¹

Department of Microbiology, University of Michigan Medical School, Ann Arbor, Michigan

Antigenic components were solubilized from a particulate fraction of mouse lymphosarcoma 6C3HED at pH 9.0–9.5. Protein, lipid, and carbohydrate were present. Three measures of antigenicity were employed. Mouse cytotoxicity tests revealed that the tumor fraction adsorbed a greater amount of rabbit antitumor antibody than did similar fractions of normal C3H mouse lymphoid tissues. In Schultz-Dale tests, prior desensitization of immune C3H mouse uterine muscle with spleen and lymph node-thymus antigens did not eliminate a response to the tumor antigens. The immunization of C3H mice by a single injection of the tumor antigenic preparation in Freund's complete adjuvant resulted in a greater number of regressions than was observed in mice treated with adjuvant alone.

¹ Aided by a grant from the U. S. Atomic Energy Commission, Contract A. T. (11-1)-70 project 9, and by Grant CY3622, from the NIH.

² Part of a thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy to the faculty of the Department of Microbiology of the University of Michigan, 1960.

Received 4/ 5/65. Revised 7/ 9/65.