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The Chromosomes of Human Intraepithelial Neoplasia: Report of 14 Cases of Cervical Intraepithelial Neoplasia and Review¹

Department of Pathology and Obstetrics and Gynecology, College of Physicians and Surgeons, Columbia University and the Obstetrical and Gynecological Service (The Sloane Hospital) of the Presbyterian Hospital, New York, New York

Chromosome counts and karyotypes were prepared from samples of dysplasia and carcinoma in situ of the uterine cervix in 14 patients with the use of tissue culture technics. The material for initial culture was identified as neoplastic epithelium in vivo by colpomicroscopy. Then a biopsy was obtained from within this area; one half was fixed for histologic examination, and in the remaining half, the neoplastic epithelium was separated from the connective tissue, minced, and placed in culture. When chromosome counts were performed each case had a modal number of 46 and predominantly normal karyotypes, except for 1 case of dysplasia in which a marker chromosome was found.

¹ This investigation was supported by USPHS Research Grant CA-07060-02 and Fellowship 1-F3-CA-23,432-01 from the National Cancer Institute.

² USPHS Special Fellow. Present address: Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, North Carolina.

Received 6/ 4/65.