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Immunoelectrophoretic Identification of Catalase Subcomponents in the Homogenates of Rat Tissues¹

Department of Pathology, Northwestern University Medical School, Chicago, Illinois

Immunoelectrophoretic analyses of catalase in a variety of tissues from normal Sprague-Dawley rats were made with rabbit anti-rat hepatic catalase serum. Several distinctive patterns of the enzyme were identified by this means. A pattern previously described for purified hepatic catalase with 6 immunologically independent subcomponents was again recognized in one of the chromatographically separated catalase fractions of the liver (RHC-A).³ Crude homogenates of the liver also were shown to contain the 6 subcomponents of the enzyme. Various combinations of the enzyme subcomponents were observed in different tissues, while the central nervous system and the ovaries revealed no catalatic activity or precipitin reaction by immunoelectrophoresis. These observations support the belief that catalase exists in multiple molecular forms and is, to some extent, characteristic of the tissue from which it is obtained.

¹ Supported in part by USPHS Grant No. CA-06591-03; the American Cancer Society, Illinois Division, and the Cancer Research Coordinating Committee, University of California, Los Angeles. A portion of this work was carried out in the Department of Pathology, University of California, School of Medicine, Los Angeles, California, during the senior author's tenure as visiting Associate Professor of Pathology.

³ The following abbreviations are used: RHC-A, 1st chromatographic fraction or fraction A of rat hepatic catalase (5); RHC-B, 2nd chromatographic fraction or fraction B of rat hepatic catalase (5); Kat f, Katalasefähigkeit; K, 1st order reaction constant; PHC, purified rat hepatic catalase; DEAE, diethylaminoethyl cellulose.

² Career Development Awardee USPHS No. 2-K3-GM-244-06.

Received 6/16/65.