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The Effect of Temperature Change upon Inclusion-containing Renal Tumor Cells of Leopard Frogs¹

Department of Pathology, University of Colorado Medical Center, Denver, Colorado

Seven leopard frogs with known primary renal tumors were brought out of hibernation to laboratory temperature (20°-22°C). The animals were killed, 2 each at Days 1, 3, and 5, and 1 at Day 7. Previously 1 frog with a renal tumor had been studied 7 days posthibernation; thus a total of 8 animals were examined. Light microscopic examination of the tumors showed that the amount of cellular debris increased within the lumina of the tumor tubules during this period. Electron microscopic observations showed that the lumina were filled with cellular debris as well as incomplete and complete viruses. Observations on the effect of increased temperature on hibernating frogs resulting in the destruction of tumor cells which contain viral inclusions is discussed.

¹ This investigation was supported by a Viral Training Research Grant 5T1 CA 5164-01 from the NIH issued to the Department of Pathology, University of Colorado Medical Center, Denver, Colorado and ACS grant IN5G, No. 7

² Present address: Department of Anatomy, University of Colorado Medical Center, Denver, Colorado.

Received 2/ 1/66.