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The Influence of 3'-Methyl-4-dimethylaminoazobenzene on the Incorporation of Stearate-1-¹⁴C and Oleate-1-¹⁴C into Microsomal Phosphatidyl Choline and Phosphatidyl Ethanolamine¹

Departments of Pathology, Los Angeles County Harbor General Hospital, Torrance, California, and the U.C.L.A. School of Medicine, Los Angeles, California

Several groups of rats were fed either a fat-free or cholesterolcontaining diet with or without 3'-methyl-4-dimethyl-aminoazobenzene (3'-Me-DAB) for 6 weeks, after which they were given 1 dose of either stearate-1-¹⁴C or oleate-1-¹⁴C and killed at 1-, 2-, 4-, and 24-hr intervals. Microsomal phosphatidyl choline and phosphatidyl ethanolamine were isolated by thin layer chromatography and their radioactivities and fatty acid composition determined by liquid scintillation spectrometry and gas chromatography.

Administration of 3'-Me-DAB to rats on the fat-free diet caused an increase in the proportion of oleic acid and a decrease in stearic acid in phosphatidyl choline. Cholesterol in the diet together with the azo dye produced an accentuation of the fatty acid alterations. With 3'-Me-DAB, there was an increased incorporation of radioactive stearate at the early time intervals, and an increased 24-hr retention of radioactive oleate, suggesting a possible increased turnover of stearate-containing phospholipids induced by the azo dye, leaving more oleate-containing phospholipids remaining in the microsomes. The combination of cholesterol with 3'-Me-DAB produced a decreased synthesis of stearatecontaining phospholipids and a lesser decreased synthesis of oleate containing phospholipids, which may explain the altered proportions of these 2 fatty acids in rats fed this diet.

¹ This investigation was supported by a grant from the American Cancer Society.

Received 3/ 4/66.