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The Effect of Methotrexate on Chemical Carcinogenesis of Hamster Buccal Pouch¹

Department of Oral Pathology, Tufts University School of Dental Medicine, Boston, Massachusetts

Forty-eight male and female Syrian hamsters, 6 months of age, were studied for the effects of systemically injected methotrexate upon the process of chemical carcinogenesis of buccal pouch. Twelve animals (Group I) had the right buccal pouch painted 3 times weekly with a 0.5% solution of 9, 10-dimethyl-1,2-benzanthracene (DMBA) in heavy mineral oil. Twelve animals (Group 2) were painted with DMBA and also injected s.c. 3 times weekly with 0.0625 mg sodium methotrexate. Remaining animals served as control groups. In those animals given injections of methotrexate, chemically induced carcinomas of the buccal pouch appeared more rapidly than in DMBA controls. The tumors were also more anaplastic and of greater size.

¹ This study received support from NIH Grants DE 01861-02 and CA-575-18.

Received 1/21/66. Revised 4/28/66.