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Changes in RNA Synthesis Following Transfer of Sarcoma 180 Ascites Cells from the Mouse to Synthetic Medium¹

Microbiology Department, Auckland University, Auckland, New Zealand

RNA synthesis in Sarcoma 180 ascites cells growing in vivo in mice was compared to RNA synthesis in Sarcoma 180 cells following transfer to synthetic medium. Following radioactive labeling of Sarcoma 180 cells, RNA was extracted with phenol and fractionated on methylated bovine serum albumin columns. The cells appeared to experience a nutritional shift with resulting cessation of ribosomal RNA synthesis following transfer to synthetic medium. Pulse-labeling experiments with transferred cells demonstrated the synthesis of a rapidly labeled, high molecular weight RNA fraction which did not appear to be ribosomal RNA precursor. Comparisons of base compositions showed that ribosomal RNA synthesis in ascitic cells 15 hr after transfer to synthetic media was still different to that observed following growth in vivo.

¹ This work was supported in part by a grant from the British Empire Cancer Society (Auckland Division) and by USPHS Grant AI-04973.

Received 3/30/66. Accepted 6/ 8/66.