This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Doering, A. Articles by Cohen, S. S. Search for Related Content PubMed PubMed Citation Articles by Doering, A. Articles by Cohen, S. S.

Some Effects of D-Arabinosyl Nucleosides on Polymer Syntheses in Mouse Fibroblasts¹

Department of Therapeutic Research, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania

The effects of 1-ß-D-arabinofuranosylcytosine and of 9-ß-D-arabinofuranosyladenine have been studied on the growth and multiplication of mouse fibroblasts in suspended cell culture. Both compounds permit a considerable enlargement of the cells but sharply inhibit multiplication. The effects of these agents on the syntheses of DNA, RNA, and protein in these cells have also been examined. Both chemicals markedly inhibit DNA synthesis and reduce the cloning capacity of the cultures. No significant effect of either compound has been detected on RNA synthesis. A slight inhibition of protein synthesis has been observed with arabinosyladenine. After prolonged inhibition of cell multiplication by either compound, washed cells, incubated in the normal medium supplemented with thymidine-2-¹⁴C, were able to synthesize DNA at an approximately normal rate for 6 hr. After this time, DNA synthesis stopped abruptly and cell division occurred in 50–80% of the cells. It has been concluded that inhibition of DNA synthesis and of cell multiplication by D-arabinosyl nucleosides is probably not attributable to irreversible lesions in the DNA resulting from terminal addition of D-arabinosyl nucleotides.

¹ This study was supported by a Special Grant for Research from the American Cancer Society, Philadelphia Division.

Received 4/25/66. Accepted 6/24/66.