This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Green, S. Search for Related Content PubMed PubMed Citation Articles by Green, S.

The Effect of 1, 3-Bis(2-chloroethyl)-1-nitrosourea on Nicotinamide Adenine Dinucleotide Glycohydrolase of Mouse and Rat Neoplastic and Normal Tissues¹

Division of Biochemistry, Sloan-Kettering Institute for Cancer Research, and Sloan-Kettering Division of Cornell University Graduate School of Medical Sciences, New York, New York

The effect of 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) on the nicotinamide adenine dinucleotide glycohydrolase (NADase) activity of mouse and rat neoplastic tissue has been studied and the results compared with those previously reported for nitrogen mustard. The experimental tumors used were: in mice, Ehrlich ascites carcinoma, Sarcoma 180 (solid and ascites), and Taper liver tumor (solid and ascites); in rats, Walker carcinosarcoma 256, Murphy-Sturm lymphosarcoma, and THF thyroid tumor. Tissues taken from BCNU-treated and -untreated animals were liver, kidney, lung, spleen, intestine, and brain. The sensitivity of tumors to BCNU varied; for a 50% or more inhibition in growth, solid tumors required about twice as much of the agent as the ascitic tumors. BCNU increased the NADase activity in all the tumors tested when it was administered to the host animals during the period of active tumor growth. No increase in the activity of this enzyme was found in any of the non-neoplastic tissues.

¹ This investigation was supported in part by the following: Grant CA-08748 from the National Cancer Institute, NIH, and Grant P-164 from the American Cancer Society. The author was the recipient of a Research Career Development Award No. 5-K3-CA-19 453.

Received 3/ 7/66. Accepted 6/28/66.