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In Vivo Localization of Anticarcinogen Antibody in Organs of Carcinogen-treated Rats¹

Department of Biochemistry Research, Roswell Park Memorial Institute,³ Buffalo, New York

Rabbit antibody against the 2-azofluorenyl group was prepared and purified specifically by the use of a hapten-specific solid adsorbent. These antibodies, when radiolabeled and injected i.v., were shown to localize specifically in the liver and kidney of rats previously injected with 2-aminofluorene and 2-acetylaminofluorene and also in the liver and kidney of rats fed 2-acetylaminofluorene. The antibodies did not localize in the organs of untreated normal rats or of untreated rats with tumors induced by feeding 2-acetylaminofluorene. The latter rats had not received any carcinogen 0.5–1 year prior to the antibody injection. However, when such tumor-bearing rats were injected with 2-amino- or 2-acetylaminofluorene prior to antibody injection, the antibodies did localize in the apparently healthy portions of liver and kidney, but not in the tumor itself. When rats were injected with 2,7-diacetylaminofluorene, 2-amino-7-acetylaminofluorene, 2,7-diaminofluorene, 2,7-dinitrofluorene, and fluorene, they did not show any localization of the radiolabeled anti-2-azofluorene antibodies above control globulin.

The anti-2-azofluorenyl antibody was shown by radioimmunoelectrophoresis experiments to be directed against the 2-azofluorenyl group since the combination of antibody with antigen was inhibited by the presence of 2-aminofluorene and to a lesser degree by 2-acetylaminofluorene and 2-amino-7-acetylaminofluorene.

The results indicate that some components of the hepatic cell surface are able to combine with fed or injected 2-aminofluorene and 2-acetylaminofluorene and then to fix the antibody in vivo, when the antibody is injected i.v. These components are not present in the tumor.

¹ Supported in part by Contract AT(30-1)2651 from the United States Atomic Energy Commission.

² This work was done during a leave of absence from the Institute for Cancer Research, Osaka University Medical School, Osaka, Japan.

³ A unit of the New York State Department of Health.

Received 7/30/65.