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Phleomycin, an Inhibitor of Replication of HeLa Cells¹

McArdle Laboratory, University of Wisconsin, Madison, Wisconsin

The antibiotic, phleomycin, exhibited 2 actions in synchronized cultures of HeLa cells: inhibition of DNA synthesis, and prevention of cell division. The agent's main effect, blockage of cells entering mitosis, was observed in cultures in which almost all of the DNA had been replicated and was obtained with concentrations that affected DNA synthesis only slightly. Kinetic studies indicated that this phleomycin-sensitive step followed completion of DNA synthesis; microscopic evidence suggested that the agent prevented the cells from entering prophase. Phleomycin's action appears to be unique and of potential value in clarifying cellular processes essential for mitosis.

¹ The investigation was supported by USPHS Grant CA-07175 from the National Cancer Institute and by a grant from the Alexander and Margaret Stewart Trust Fund.

² The senior author is a recipient of a research career award, 5K6-CA-685-04, from the USPHS.

Received 5/ 5/65. Revised 8/ 9/65.