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Studies on Ehrlich Ascites Cells Exposed to the Sodium Salt and Ethyl Ester of Heptyloxyacetic Acid-1-¹⁴C

Division of Microscopic Anatomy, Ontario Veterinary College, and the Department of Apiculture, Ontario Agricultural College, Guelph Canada

Autoradiographic and cytologic studies were undertaken on Ehrlich ascites tumor cells exposed to ethyl heptyloxyacetate-1-¹⁴C and sodium heptyloxyacetate-1-¹⁴C. These ascites cells exposed to the water-soluble compound sodium heptyloxyacetate showed no changes from the controls, whereas those exposed to the lipid-soluble compound ethyl heptyloxyacetate exhibited a variety of changes, including stretching and clumping of metaphase chromosomes, followed by a mitotic suppression for approximately 48 hr after treatment. The autoradiographs of ethyl heptyloxyacetate-treated samples showed a maximum incorporation in the cells at 1 hr after treatment, whereas those of sodium heptyloxyacetate showed no accumulation of grains in the cells in any of the samples, suggesting either rapid breakdown in the peritoneal fluid or failure to cross the cell membrane.

The chromosome features of the modal cells in the control and sodium heptyloxyacetate-treated mice were identical, whereas all ethyl heptyloxyacetate-treated mice differed from the controls and from one another. The predominant cell types of all 6 ethyl heptyloxyacetate-treated mice exhibited hypotetraploid numbers.

Received 6/17/65.