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Nitrosamines as Environmental Carcinogens

II. Evidence for the Presence of Nitrosamines in Tobacco Smoke Condensate

Department of Organic Chemistry, University of Pretoria, Pretoria, South Africa

Trace amounts of N-nitrosamines in biologic material were determined qualitatively and semiquantitatively by conversion of the nitrosamines into the corresponding asymmetrical hydrazines. The hydrazines were selectively concentrated by extraction technics and identified by means of thin layer chromatography after reaction with 4-nitro-azobenzene-4'-carboxylic acid chloride.

Received 5/25/65. Revised 9/20/65.