This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Slotnick, I. J. Articles by James, D. H. Search for Related Content PubMed PubMed Citation Articles by Slotnick, I. J. Articles by James, D. H., Jr.

Vincristine Inhibition of DNA Synthesis in Tetrahymena pyriformis¹

Laboratory of Bacteriology, St. Jude Research Hospital and Department of Microbiology, University of Tennessee School of Basic Medical Sciences, Memphis, Tennessee

The effect of vincristine sulfate (VCR)² on the incorporation of isotopic precursors into protein, RNA, and DNA of the protozoan Tetrahymena pyriformis has been studied. DNA synthesis in logartithmically growing cells of T. pyriformis was almost completely inhibited by concentrations of VCR which only moderately depressed RNA and protein synthesis. Under appropriate conditions this selective inhibition of DNA synthesis may be prevented by riboflavin or flavin mononucleotide. Flavin adenine dinucleotide was without effect.

¹ Supported by USPHS Research Grant No. CA 07295-02.

² The following abbreviations are used: VCR, vincristine sulfate, TCA, trichloracetic acid; FMN, flavin mononucleotide; VLB, vinblastine.

Received 6/ 1/65. Revised 9/13/65.