This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Kitagawa, M. Articles by Pressman, D. Search for Related Content PubMed PubMed Citation Articles by Kitagawa, M. Articles by Pressman, D.

Carcinogen-binding Antigens in Rat Liver Microsomes¹

From the Department of Biochemistry Research, Roswell Park Memorial Institute,⁴ Buffalo, New York

Microsomal fractions were prepared from livers of normal rats and rats treated with 2-aminofluorene or 2-acetylaminofluorene as well as from hepatomas induced by feeding 2-acetylaminofluorene. The microsomes were solubilized with deoxycholate and examined by immunoelectrophoresis using rabbit antiserum against normal liver microsomes which had been absorbed with rat plasma and kidney microsomes.

At least 9 precipitate arcs were detected with preparations from livers of normal rats. Similar patterns were obtained with preparations from livers of rats which had been injected with 2-aminofluorene or 2-acetylaminofluorene 72 hr earlier. In the latter case, at least 2–3 arcs were shown to fix anti-2-azofluorene-¹²⁵I antibody, indicating that some microsomal components combined with the injected carcinogen.

Some of the liver microsomal antigens including one of the carcinogen-binding components were not detected in preparations from livers of rats which had been fed 2-acetylaminofluorene for 16 weeks and removed from the carcinogen diet for 1, 2, or 4 weeks.

Similar but more extensive changes in antigen composition were observed with preparations from hepatomas. Some antigens, including the carcinogen-binding components, were not observed. In contrast, some antigens not clearly detectable in normal liver preparations were observed in hepatoma preparations.

The role of the binding of carcinogen to the microsomal components and the role of these components themselves in carcinogenesis remain to be seen.

¹ Supported in part by Contract AT (30-1) 2651 from the U. S. Atomic Energy Commission and Grant 242-C from the American Cancer Society.

² On leave from the Institute for Cancer Research, School of Medicine, Osaka University, Osaka, Japan (present address).

³ On leave from the Institute of Cancer Immunopathology, Hokkaido University, Sapporo, Japan.

⁴ A unit of the New York State Department of Health.

Received 8/19/65. Revised 11/19/65.