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Effect of Glucose and Succinate on Respiration in Fresh and Incubated Ascites Tumor Cells^{1 ,2}

From the Virus Laboratory, Department of Microbiology, The Pennsylvania State University, University Park, Pennsylvania

Respiration was measured in ascites tumor cells which had been incubated for various lengths of time in a phosphate-buffered saline or ascites fluid at 37°C and 5°C. The oxidation of glucose was inhibited by 33% following incubation in the phosphate-buffered saline for 45 min at 37°C, while oxidation of succinate was accelerated. Similar results were obtained with cells incubated in phosphate-buffered saline at 5°C. Incubation of the tumor cells at 37°C in phosphate-buffered saline for 45 min resulted in a loss of 20% of the cellular acid-soluble ribonucleotides to the environment. Incubation of the ascites tumor cells in ascites fluid delayed the onset of these effects.

¹ Supported in part by predoctoral (B. H.) and postdoctoral (F. Z.) traineeships under Training Grant 5T1-GM-512, from the Institute of General Medical Sciences, NIH.

² Paper No. 2815 in the journal series of the Pennsylvania State University Agricultural Experiment Station.

³ Present address: Department of Pathology, University of Colorado Medical Center, 4200 East Ninth Ave., Denver, Col.

⁴ Present address: Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.

Received 8/ 9/65. Revised 11/24/65.