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In Vitro Reactions of Lymphocytes from Normal Mice and from Viral and X-Ray-induced Lymphomas¹

Tumor Research Laboratory, Research and Surgical Services, Veterans Administration Hospital, Hines, Illinois

The in vitro reactions of lymphocytes from normal spleen and thymus of mice were compared with the reactions of cells from a viral lymphoma and from X-ray-induced lymphomas. The normal murine lymphocytes survived a shorter time than human lymphocytes but survived longer in vitro than the cells of mouse lymphoma. Mitoses were more numerous in the suspensions from the lymphomas than in normal lymphocyte suspensions. Some of the cells from both normal spleen and viral lymphomas underwent enlargement and transformation into lymphoblastoid cells. In general, the normal and neoplastic lymphocytes were sensitive to X-rays (1000 r) and to prednisolone (10 µg). However, the cells from some of the X-ray-induced lymphomas were resistant to X-rays or prednisolone or both. A positive correlation was obtained between resistance to the 2 reagents. In general, both normal and neoplastic lymphocytes survived longer when incubated at 27 and 15 than at 37°C. However, the cells from some of the X-ray-induced lymphomas survived relatively poorly at the low temperatures. It seemed that the X-ray-induced lymphomas were heterogeneous in the in vitro reactions of their cells to X-rays, prednisolone, and low temperatures.

¹ Aided by Grant CA 06631 from the National Cancer Institute, USPHS.

Received 10/15/65.