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Respiration Capacity of Transplantable Rat Leukemic and Endocrine-dependent Tissue Tumor Systems¹

Mason Research Institute, Worcester, Massachusetts

The respiration capacity of 3 types of rat transplantable leukemias with solid masses and 2 endocrine target organ neoplasms was explored. The oxygen consumption and ability to reduce triphenyltetrazolium in the presence of dehydrogenase substrates was determined on tissue slices of tumors, livers, and spleens from tumor-bearing rats and mammaries, prostates, livers, and spleens from normal rats. It was found that leukemic tissue had a depressed endogenous oxygen uptake and dehydrogenase activity. Succinate introduction improved oxygen consumption in LW/12 leukemia and R-35 mammary adenocarcinoma tissue modestly and markedly in livers from tumorous and normal rats. Spleens and prostates from either group of animals were poor responders. Additions of succinate, isocitrate, malate and -ketoglutarate elicited increments in formazan production, indicating a functioning cytochrome chain for neoplastic and control tissues. Livers from leukemic animals responded remarkably to succinate, while 11095 prostatic carcinoma and its homologous control yielded a nearly identical response to all substrates. The oxygen uptake pattern for the 5 tumor systems remained fairly reproducible over many transplant generations.

¹ Supported by Contract pH43-65-6 from the Cancer Chemotherapy National Service Center, National Cancer Institute, USPHS.

Received 10/20/65.