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Metabolism of Deoxyribonucleotides

II. Enzymatic Phosphorylation of Deoxycytidylic Acid in Normal Rat Liver and Rat Ascites Hepatoma Cells

Department of Biochemistry, Institute for Virus Research, Kyoto University, Kyoto, Japan

Enzyme levels concerning deoxy- and ribonucleoside triphosphate synthesis were determined in normal rat liver and dimethylaminoazobenzene-induced rat ascites hepatoma cells (AH13).

In hepatoma cells, dAMP,¹ AMP, dGMP, GMP, and dTMP were more extensively phosphorylated than in normal liver, but in marked contrast, phosphorylation of dCMP and CMP was restricted. The levels of enzyme activities catalyzing the phosphorylation of dTDP were exceedingly higher than those of monophosphokinases even in normal liver as well as in hepatoma cells. Evidence is presented which suggests that the low level of dCMP and CMP kinase activities in hepatoma cells may probably be a consequence of the low concentration of thiol compound(s) in these cells.

¹ The abbreviations used are: dAMP and AMP, deoxyadenoines and adenosine 5'-monophosphate, respectively; dGMP and GMP, deoxyguanosine and guanosine 5'-monophosphate; dCMP and CMP, deoxycytidine and cytidine 5'-monophosphate; UMP, uridine 5'-monophosphate; dTMP, dTDP, and dTTP, deoxythymidine 5'-mono-, di-, and triphosphate; P_i, inorganic orthophosphate; PP_i, inorganic pyrophosphate; Tris, tris(hydroxymethyl)aminomethane; ATP, adenosine triphosphate.

Received 9/20/65. Revised 1/ 3/66.