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Antigenic Relationship of Mouse Rhabdomyosarcoma to Human Rhabdomyosarcoma and to Human and Mouse Muscle¹

Laboratory of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee³

A study was made to confirm immunohistochemically that a transplantable mouse rhabdomyosarcoma was of muscle origin. Antiserum developed in rabbits against the mouse tumor was tested by the Ouchterlony agar diffusion method and by the indirect fluorescent antibody procedure.

Antibodies to soluble antigens were shared by mouse spleen, kidney, lung, and mouse serum. The removal of these by specific absorption with mouse tissues left antibodies which were directed against particulate muscle elements. The results confirm the presence of normal muscle antigens in the mouse rhabdomyosarcoma. Rabbit antihuman myosin, serum which cross reacts with mouse muscle, gave essentially the same results when tested against the mouse tumor.

Using the technics and antisera employed in this study, a tumor-specific antigen could not be demonstrated in the mouse rhabdomyosarcoma. When compared to normal mouse muscle, some antigenic deficiency was indicated in the insoluble antigenic make up of the mouse tumor.

¹ This work was supported in part by the American Lebanese Syrian Associated Charities (ALSAC).

² Present address: Department of Periodontology, College of Dentistry, University of Tennessee, Memphis, Tennessee.

³ Affiliated with the University of Tennessee Medical Units, Memphis, Tennessee.

Received 10/ 4/65. Revised 1/14/66.