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Differences between the Microsomes of Normal Rat Liver and of N-2-Fluorenylacetamide-induced Rat Hepatoma as Determined by the Paired Label Antibody Technic¹

Department of Biochemistry Research, Roswell Park Memorial Institute,³ Buffalo, New York

Rabbit antibodies against the microsome fractions of a chemically induced rat hepatoma and of normal rat liver were compared by the paired label radioantibody technic. The antihepatoma antibodies were labeled with ¹²⁵I and the anti-normal-liver antibodies were labeled with ¹³¹I. The 2 were mixed and then injected or treated in vitro simultaneously. The 2 antibodies differed in vivo in their localizing properties in hepatoma and in normal liver. They were fractionated in vitro into fractions containing hepatomaphilic or normal-liver-philic antibodies. The results indicate differences in the antigen composition between the microsome fractions of the 2 tissues as well as differences in the antibody composition between the antisera produced against these preparations.

¹ Supported in part by Contract AT-2651 from the U.S. Atomic Energy Commission and Grant T-242 from the American Cancer Society.

² This work was done during a leave of absence from the Department of Obstetrics and Gynecology, Osaka University Medical School, Osaka, Japan (present address).

³ A unit of the New York State Department of Health.

Received 8/19/65. Revised 1/20/66.