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Aging and Cancerigenesis

III. Effect of Age on Isoantibody Formation¹

Division of Experimental Chemotherapy, Sloan-Kettering Institute for Cancer Research, New York, New York

In continuance of the investigation of the possible interrelationship among aging, immunity, and cancerigenesis, isoantibody formation was studied in young and old mice implanted with the homologous EL4 ascites leukemia. The production of isoantibodies was determined weekly in pooled sera using the cytotoxic antibody test and the opsonization technic involving phagocytosis of tumor cells by peritoneal macrophages in vitro following opsonization with isoantibody. Each young adult female Swiss ICR/Ha mouse (3 months old) completely rejected an intradermal implant of 5 x 10⁵ EL4 cells, whereas progressive growth took place in 55% of the 18-month-old female breeders. Relatively high titers of isoantibody were produced in young mice and to a significantly lesser degree in old mice, especially in those succumbing to progressive tumor growth. This difference in isoantibody formation in old and young mice occurred again after a 2nd implantation (i.p.) of 2 x 10⁶ EL4 cells. The importance of these differences is discussed.

¹ This work was supported in part by grants from the American Cancer Society, Inc., and by Grants T4CA-5015 and CA 08748 from the National Cancer Institute, National Institutes of Health, USPHS.

² Visiting Investigator.

Received 1/ 7/66.