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Chemical, Enzymatic, and Cytochrome Assays of Microsomal Fraction of Hepatomas with Different Growth Rates

Biochemistry Division, National Cancer Center Research Institute, Tsukiji, Chuo-ku, Tokyo, Japan, and the Laboratory of Biochemistry, National Cancer Institute, NIH, USPHS, Bethesda, Maryland

The microsomal fractions from hepatomas of different growth rates have been analyzed for cytochrome components, enzymatic activities, and some chemical constituents. Regenerating liver and embryonal liver were assayed in the same way for comparison. The hepatomas examined included minimal deviation type Morris hepatomas 7793, 7794A, 7795, and 7316A, and fast-growing Yoshida hepatomas AH 130 and AH 371.

Microsomal cytochromes, cytochromes b₅ and P-450, and the microsomal enzymes glucose-6-phosphatase, sulfatase C, NADH₂-cytochrome b₅ oxidoreductase, NADH₂-cytochrome c oxidoreductase, NADH₂-cytochrome c oxidoreductase, and aromatic hydroxylating activity have been found in minimal deviation hepatomas at equivalent or somewhat lower levels than those of normal or regenerating liver. In Yoshida hepatomas AH 130 and AH 371, and in embryonal liver, these cytochromes and enzymes were extremely depressed or deleted. The decrease in activity in these components correlated roughly with increasing rates of growth. Magnesium-activated adenosine triphosphatase in the microsomal fraction was elevated in all hepatomas examined.

The RNA content in the microsomal fractions increased while phospholipid content decreased with increasing growth rate.

Received 12/13/65. Revised 3/28/66.