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Effects of Actinomycin D on Avian Myeloblast and BAI Strain A Virus RNA Synthesis in Vitro¹

Department of Surgery, Duke University Medical Center, Durham, North Carolina

Avian myeloblasts infected with BAI strain A avian tumor virus were cultured in vitro and treated with actinomycin D. The effects of the drug on cell growth and death and the synthesis of cell and virus RNA were examined. Actinomycin D (AD) concentrations of 0.01–0.05 µg/ml caused cell damage and death at proportional rates, and, as shown in earlier work, inhibited cell RNA synthesis up to 60% without depressing virus RNA synthesis or virus particle liberation. In comparison, 0.5 µg AD/ml caused more rapid cell death with parallel inhibition of 70–85% of cell RNA synthesis and 70% of virus RNA synthesis but did not decrease the output of particles having the characteristic virus adenosinetriphosphatase activity. These results are discussed and compared with findings with other virus-host systems.

¹ This work was aided by a research grant (C-4572) to Duke University from the National Cancer Institute, NIH, USPHS; by a grant from the American Cancer Society, Inc. (E-84A); and by the Dorothy Beard Research Fund.

Received 2/ 7/66.