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The Effect of Antibody to L-Phenylalanine Mustard Conjugate on Malignant Cells Selectively Marked through "Early Inflammatory-like" Vascular Permeability¹

Departments of Surgery and Medicine, Harvard Medical School, and the Massachusetts General Hospital, Boston, Massachusetts

Antibody was produced against the haptene L-phenylalanine mustard by conjugating the mustard with human -globulin and using the conjugate plus Freund's adjuvant to immunize rabbits. In mice with transplanted ependymomas, the haptene L-phenylalanine mustard was concentrated in the region of the tumor cells by virtue of the "early inflammatory-like" permeability of tumor vessels to the mustard. Malignant ependymomas in mice treated by giving the mouse an injection of a minimal dose of L-phenylalanine mustard followed in 0.5 hr by specific antibody showed decreased growth over the control groups. Haptene-specific antibody was shown by fluorescent technics to localize in the region of the cell membrane of L-phenylalanine mustard-treated tumors.

¹ This investigation was supported by Grant Al-02392 from the National Institutes of Allergy and Infectious Diseases, CA-07368 from the National Cancer Institute, NIH, and from the American Cancer Society.

Received 11/ 9/65. Accepted 4/14/66.