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Studies on Latent Derivatives of Aminoethanethiols as Potentially Selective Cytoprotectants

I. A Spectrophotometric Assay for Aminoethanethiols in Biologic Media¹

Collaborative Research, Inc., Waltham, Massachusetts

A convenient spectrophotometric method for determination of aminoethanethiols in biologic materials is described. The method is based on the reaction of aminoethanethiols with 3-fluoropyruvate at pH 9 to produce products that absorb at 300 mµ. Disulfides formed by oxidation of aminoethanethiols can be determined after their reduction by dithiothreitol (or dithioerythritol) or cleavage with bisulfite. The method is suitable for studies of "latent" protective compounds that can be converted by various tissues to aminoethanethiols.

¹ Supported by Research Contract PH43-62-170, Cancer Chemotherapy National Service Center, National Cancer Institute, NIH, Bethesda, Md.

² Present address: Department of Biological Chemistry, Harvard Medical School, Boston, Mass.

³ Department of Biochemistry, Tufts University School of Medicine, Boston, Mass.

Received 5/ 3/66. Accepted 8/19/66.