This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hotham-Iglewski, B. Articles by Ludwig, E. H. Search for Related Content PubMed PubMed Citation Articles by Hotham-Iglewski, B. Articles by Ludwig, E. H.

The Cytocidal Action of Normal Human Serum on Ehrlich Lettré Ascites Tumor Cells

II. Biochemical Studies

Virus Laboratory, Department of Microbiology, The Pennsylvania State University, University Park, Pennsylvania

Incubation of Ehrlich Lettré ascites tumor cells with normal human serum at 37°C was found to result in the loss of a high percentage of cellular acid-soluble ribonucleotides, a decrease in lactic acid production, and a lower rate of endogenous respiration than in controls. Oxidation of succinate, -glycerophosphate, and -ketoglutarate was stimulated in cells preincubated in normal human serum. Furthermore, it was possible to obtain respiratory stimulation with adenosine-5'-diphosphate with several substrates employing whole tumor cells preincubated in normal human serum. Respiratory activity of homogenates of cells preincubated in normal human serum was equivalent to that of homogenates of control tumor cells.

¹ Predoctoral Trainee, Research Training Grant 5T1 GM-512, Institute of General Medical Sciences, NIH.

² Present address: Department of Virology, The Public Health Research Institute of the City of New York, Inc., New York, N.Y.

Received 2/17/66. Accepted 8/18/66.