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Neutralization of Sublines of a Mouse Leukemia Virus with Murine Antibody, as Measured by the Spleen Focus Assay¹

Departments of Medicine, Pathology, and Epidemiology, The University of Texas, M. D. Anderson Hospital and Tumor Institute, Houston, Texas

The spleen focus assay has been applied for the neutralization of sublines of the Rauscher virus. Murine immune sera, produced by photodynamically inactivated stock preparations of the virus, and by live attenuated tissue culture virus, were used. Dilutions of 1:4–1:8 of an immune serum produced by the killed virus inhibited 75.6–98.4% of the focus-producing potency of both stock and control virus preparations obtained from untreated leukemic mice. The same serum dilutions failed to inhibit more than 1–31% of the focus-producing potency of Rauscher virus preparations obtained from leukemias that recurred after irradiation treatment and adoptive immunization. Dilutions of 1:2 of immune sera produced by live leukemia virus neutralized significantly, but not completely, the latter sublines of the Rauscher virus.

These findings suggest that (a) the spleen focus assay can be applied to virus-neutralization tests and that (b) the avidity of a murine leukemia virus to neutralizing antibody may change during various phases of the disease.

¹ Supported by USPHS Research Grants CA-07923 and CA-06939 and Award 5-K3-CA-16747.

Received 6/ 6/66. Accepted 7/21/66.