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Comparison of Direct and Short-term Tissue Culture Technics in Determining Solid Tumor Karyotypes¹

Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06510

Two short-term tissue culture technics for determining the chromosome complement of solid human tumors are described. The simplest and most effective technic involves culturing minced tumor tissue for 1–3 days, converting the minced tumor to a suspension of cells by shaking, and harvesting this suspension essentially as in lymphocyte cultures. The technics provide increased numbers and quality of metaphase mitoses for study in comparison to the direct preparations and can be applied to a wide range of tumors. Cell line selection was not demonstrated by the available data, but more data are needed before it can be excluded. Short-term tissue culture technics represent extremely valuable tools in studying the cytogenetic and other aspects of human neoplasms.

¹ This project was supported in part by Summer Fellowship ACA-IN-31, F-913, USPHS Career Development Award K3-CA-19, 745 from the National Cancer Institute, Research Grant GM 11078-03 from the General Medical Science Branch, and the John A. Harford Foundation.

² This project was submitted in partial fulfillment of the requirements for the degree of Doctor of Medicine.

Received 2/ 6/67. Accepted 6/ 6/67.