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Localization of Tumor Antigens in Adenovirus-12-induced Tumor Cells and in Adenovirus-12-infected Human and Hamster Cells by Ferritin-labeled Antibodies¹

Department of Biology, McMaster University, Hamilton, Ontario, Canada, and Viral Oncology Section, Roswell Park Memorial Institute, Buffalo, New York

Electron microscopic studies revealed bundles of fibers in nuclei of Adenovirus type 12 (Ad. 12)-infected human amnion cells (AV-3), in nuclei of hamster cells infected in vitro with Ad. 12, and in the cytoplasm of Ad. 12-induced neoplastic cells from Syrian hamsters. These bundles of fibers react with ferritinlabeled T-antibodies obtained from Ad. 12-tumor-bearing hamsters. The bundles of fibers seen in Ad. 12-infected hamster cells and in Ad. 12-induced neoplastic cells appear to be antigenically and morphologically analogous to structures observed in Ad. 12-infected AV-3 cells. The bundles of fibers could be considered to be "viral footprints." In neoplastic cells they may serve as indicators of viral etiology.

¹ This work was supported in part by the National Cancer Institute of Canada and in part by Grant CA-07745 from the USPHS.

Received 5/31/66. Accepted 6/12/67.