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Tumor Production in Vivo and Cell Transformation In Vitro by Two SV20 Strains¹

Department of Microbiology, School of Medicine, University of Pittsburgh, and the Departments of Surgical Pathology and Virology, William H. Singer Memorial Research Institute of the Allegheny General Hospital, Pittsburgh, Pennsylvania

Two serologically distinguishable SV20 strains induced a high incidence (43 to 92%) of tumors in hamsters and were found to transform BHK-21 cells. Both virus strains produced a common tumor antigen, serologically distinct from SV40 tumor antigen. Its synthesis in infected tissue culture cells could be demonstrated by complement fixation and fluorescent antibody technics. Both strains induced a similar type of tumor in which the predominating pattern was the "fibrosarcoma" type, although areas suggesting a "lymphoma" or other types were also seen. Transformation of BHK-21 cells was obtained in cultures inoculated either as cell monolayers or as freshly trypsinized cell suspensions. The transformation was characterized by morphologic alteration of the cells and the development of SV20 tumor antigen without any detectable infectious virus. These cells did not react by fluorescent antibody tests with SV40 tumor antisera.

¹ Supported by NIH Grant No. AI-02535.

² Present address: Department of Microbiology, G. D. Searle and Co., Chicago, Illinois 60680.

Received 3/ 3/67. Accepted 6/12/67.