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Hybridization of a Malignant Melanoma Cell Line with L Cells in Vitro¹

Department of Obstetrics and Gynecology, Cornell University Medical College, and the Sloan-Kettering Institute for Cancer Research, New York, New York 10021

Cellular and nuclear fusion between two cell lines, each derived from a different inbred mouse strain, resulted in a "hybrid" line which is capable of continuous propagation. The parent cell lines were a mouse melanoma line and a subline of L cells which did not produce tumors when injected into C3H mice. The hybrid cells contain the sum of the chromosomes of both parents, both in kind and number, with genes of both parents active. The hybrid cells inherited from the C57BL melanoma parent the capacity for proliferative growth in vivo, genes for H-2^b histocompatibility antigens, and the ability to produce inosinic acid pyrophosphorylase. From the C3H parent, they inherited genes for H-2^k histocompatibility antigens and a possible suppressor of melanin production. Capacity for progressive growth in vivo appears to be "dominant" in the hybrid; the tumors produced by injecting cloned hybrid cells into F₁ hybrid mice are transplantable, and have been carried for 11 transplant generations over a period of 11 months. The chromosome number of the hybrid tissue culture cells has remained virtually constant in 500 cell generations of in vitro culture.

¹ This investigation was supported in part by USPHS Grants CA 10095-01 and HD-00635. A preliminary report was presented (and abstracted) at the Third International Congress of Human Genetics, September 1966 (p. 92).

Received 3/28/67. Accepted 6/15/67.