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Some Properties of ß-Glucuronidase Activity in Normal Rat Liver and in Several Transplantable Rat Hepatomas¹

Division of Biological Sciences, Biochemistry and Biophysics Section, University of Connecticut, Storrs, Connecticut 06268

The distribution and activity of ß-glucuronidase in several transplantable tumors have been compared with those of normal rat liver. In all the tumors studied, specific enzyme activity ranged from 20% to 50% that of liver. In rat liver, 5% of the total enzyme activity was recovered in the high-speed supernatant fraction, while in the tumors it ranged from 11 to 38%. The distribution of free and total enzyme in the four subcellular fractions of the Morris 5123 D and Novikoff hepatoma was also compared to rat liver. In addition to increased activity in the supernatant fraction, both tumors had a lowered percentage of total activity in the microsome fraction. The Novikoff hepatoma, in addition, had increased activity in the nuclear fraction and a decrease in the mitochondrial fraction.

In vitro experiments in which mitochondrial fractions or homogenates were incubated or treated with various chemicals suggested that the stability of those hepatoma lysosomes studied varied among themselves and differed as well from that of normal rat liver.

¹ Supported by Grant P-303B from the American Cancer Society and CA-07824 from the National Cancer Institute, NIH.

² Abstracted from a thesis presented in partial fulfillment of the requirements for the Ph.D. degree, University of Connecticut, 1966. Present address: Noble Research Foundation, Ardmore, Oklahoma.

³ Supported by Career Award 31–63 from the National Cancer Institute, NIH.

Received 1/24/67. Accepted 6/22/67.