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Studies on the Relationship of Hemadsorption by Normal Murine Spleen and Murine Tumors¹

Department of Surgery, State University of New York at Buffalo, Buffalo, New York, and the Department of Surgery and Cell Biology, University of Kentucky Medical Center, Lexington, Kentucky 40506

The relationship of hemadsorption by methylcholanthrene-induced sarcoma (MC-Sa) and normal murine spleen was studied. Since the hemadsorbing factor of spleen and MC-Sa sections appeared identical, pathologic lesions were created in mice, and these were found to hemadsorb the same species' erythrocytes that are adsorbed by spleen and MC-Sa. In further studies, spleen and MC-Sa sections from mice preimmunized with either bovine or porcine erythrocytes were found to adsorb those species' erythrocytes used for preimmunization. Although human erythrocytes treated by receptor-destroying enzyme of Vibrio comma (RDE) were not adsorbed to spleen and MC-Sa sections, bovine and porcine erythrocytes treated with RDE were adsorbed to spleen and MC-Sa sections obtained from preimmunized mice. Significant differences were also noted between the hemadsorbing factor and the hemagglutination in preimmunized mice. By washing sections of spleen or MC-Sa obtained from preimmunized mice, it was found that human erythrocytes were still adsorbed while bovine or porcine erythrocytes were not adsorbed. In addition, primary cell cultures of MC-Sa from mice preimmunized with bovine or porcine erythrocytes adsorbed human erythrocytes but did not adsorb bovine or porcine erythrocytes. Furthermore, studies on the different tumor fractions of MC-Sa obtained from preimmunized mice showed that, while the reaction with human erythrocytes was found only in the heavy fraction, the reaction with the species' erythrocytes used for preimmunization was present in the supernatant after centrifugation at 105,000 x g. It would appear from these studies that hemadsorption detected in MC-Sa is not due merely to the infiltration by MC-Sa of hemadsorbing spleen cells.

¹ This work was supported in part by USPHS Grant No. 1 SO1 FR-05400-04 (State University of New York at Buffalo) and is in part a report of work done under contract [No. 12-14-100-8141 (73)] with the U. S. Department of Agriculture and authorized by the Research and Marketing Act of 1946. The contract is being supervised by the Eastern Utilization Research and Development Division of the Agricultural Research Service (University of Kentucky Medical Center).

Received 1/23/67. Accepted 6/29/67.