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Studies on Passage of the Hemadsorbing Factor of Murine Tumors to Normal Cell Cultures¹

Department of Surgery, State University of New York at Buffalo, and the Departments of Surgery and Cell Biology, University of Kentucky Medical Center, Lexington, Kentucky 40506

The hemadsorbing factor of methylcholanthrene-induced sarcoma (MC-Sa) was studied in passage experiments. Frozen and thawed suspensions of MC-Sa excised from mice or grown in cell culture were inoculated into tubes containing primary mouse embryo tissue culture (METC). After incubation for three to five days the contents of these tubes were frozen and thawed and passed at a dilution of 1:10 into a new primary METC. Positive hemadsorption tests were obtained after five passages of the MC-Sa-inoculated METC. Passage controls of METC and uninoculated METC controls failed to hemadsorb. Surprisingly, METC inoculated with frozen and thawed suspension of murine kidney also hemadsorbed after five passages, while METC inoculated with a similar preparation of melanoma failed to hemadsorb. In addition, METC inoculated with this MC-Sa preparation and tested for hemadsorption demonstrated a growth curve similar to that seen in viral studies. These studies suggest that a virus-like agent is associated with the hemadsorption by MC-Sa. Furthermore, it would appear that this agent is present in normal murine kidney and unmasked during passage to METC. The relationship of this hemadsorbing agent to carcinogenesis is not defined by these experiments. Consequently, studies are underway to evaluate the interrelationship of carcinogens and this hemadsorbing factor.

¹ This work was supported in part by USPHS Grant No. 1 SO1 FR-05400-04 (State University of New York at Buffalo) and is in part a report of work done under contract [No. 12-14-100-8141 (73)] with the U. S. Department of Agriculture and authorized by the Research and Marketing Act of 1946. The contract is being supervised by the Eastern Utilization Research and Development Division of the Agricultural Research Service (University of Kentucky Medical Center).

Received 1/23/67. Accepted 6/29/67.