This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Pamukcu, A. M. Articles by Goksoy, S. K. Search for Related Content PubMed PubMed Citation Articles by Pamukcu, A. M. Articles by Goksoy, S. K.

Influence of a Papilloma Vaccine on Chronic Bovine Enzootic Hematuria¹

Department of Pathological Anatomy, College of Veterinary Medicine, University of Ankara, Ankara, Turkey, and the Department of Veterinary Science, University of Wisconsin, Madison, Wisconsin 53706

The effect of repeated injections of a formalinized wart vaccine on development of urinary bladder tumors of cattle has been studied in the area of Bolu, Turkey, where such bovine tumors are common. The vaccine consisted of a 5 percent suspension of bovine wart tissue with formalin to make a final concentration of 0.5 percent and was injected intradermally in the caudal fold of the tail with 0.5 ml at each of two sites. The vaccine was not completely inactivated since fibropapillomas developed at the site of injection in some animals. Six hundred sixty-five calves received two vaccine injections one month apart when 6 to 12 months old, and those available were revaccinated every six months. Three hundred twenty-seven cattle served as non-vaccinated controls and received a placebo of distilled water instead of vacine. In one group, 8 percent of vaccinated animals (32/399) and 4.5 percent of nonvaccinated cattle (9/196) developed hematuria within four years after the first vaccination. In another group, 3 percent of vaccinated (8/266) and 1.5 percent of nonvaccinated (2/131) developed hematuria within less than 3 years. Seven animals with hematuria were killed to study the pathology of the bladder. The lesions resembled the early stage of naturally occurring bladder tumors which were common in the area. The pathologic changes consisted of hyperplasia, glandular metaplasia and transitional cell papilloma. They varied with the duration of hematuria.

The wart vaccine did not prevent development of tumors in the urinary bladder and may have actually enhanced the incidence of tumors.

¹ This investigation was supported by Grant No. FG-Tu 102 of the U. S. Department of Agriculture, Agricultural Research Service, Grant from WHO, and NIH Grant CA 04627.

² Department of Pathological Anatomy, College of Veterinary Medicine, University of Ankara, Ankara, Turkey.

³ Department of Veterinary Science, University of Wisconsin, Madison, Wisconsin.

Received 4/28/67. Accepted 7/12/67.