This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Heneen, W. K. Articles by Nichols, W. W. Search for Related Content PubMed PubMed Citation Articles by Heneen, W. K. Articles by Nichols, W. W.

Cell Morphology of a Human Diploid Cell Strain (WI-38) after Treatment with Arabinosylcytosine¹

South Jersey Medical Research Foundation, Camden, New Jersey

Cell morphology was studied in a human diploid cell strain (WI-38) treated with various concentrations of arabinosylcytosine for different periods. Mitotic inhibition followed most of these treatments except for the short treatments with the lowest concentration, which allowed mitosis in the postreatment growth period. Paired acentric fragments were seen in anaphases after these treatments.

The treated cells exhibited certain morphologic characteristics, such as clustering of cells, appearance of oval, elongated nuclei and cells, increased cytoplasmic granularity, increased size of nuclei and nucleoli, fusion and vacuolation of nucleoli which became darker, and organization of new nucleoli and decreased stainability of the reticulum. The changes in the appearance of the nucleoli may indicate increased metabolic activities. Many of these morphologic characteristics resemble changes seen in transformed cells.

¹ This work was supported in part by Grants CA-03845 and CA-04953 from the NIH and by Research Career Development Award 5-K3-CA-16.749.

² Institute of Genetics, University of Lund, Lund, Sweden.

Received 1/17/66. Accepted 9/ 1/66.