This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Miyasaki, K. Articles by Fujita, T. Search for Related Content PubMed PubMed Citation Articles by Miyasaki, K. Articles by Fujita, T.

On the Phagocytizing Activities of Ehrlich Ascites Tumor Cells

Department of Pathology, Division II, School of Medicine, Kobe University, Kobe, Japan

Ehrlich ascites tumor cells actively phagocytize the pigments derived from benzidine yellow. The pigments were synthesized from acetoacet-arylide, and 5 derivatives with similar chemical structure were prepared by conjugation of various substitutes. Polyoxyethylene lauryl ether (a), polyoxyethylene nonylphenol ether (b), conjugated material of naphthaliosulfonic acid and formaldehyde (c), polyoxyethylene alkyl ether (d) polyoxyethylene alkylphenol ether (e), and a mixture of c and e (f); were used as solvents. Two-tenths ml of 0.5–1.5% of these pigment solutions (0.1% in water) were injected into the peritoneal cavities of mice on the 4th and 5th day after transplantation of Ehrlich ascites tumor cells. Phagocytizing activity was studied on the 7th day after the transplantation of the tumor cells. A higher rate of phagocytosis appeared in animals injected with pigments which contained —CH₃ substituted on the benzidine base; a lower rate of phagocytosis appeared when pigments with —Cl were used. This fact indicates that efficiency of phagocytosis is dependent upon minute differences in chemical structure. Phagocytizing activity is also affected by the type of solvent, and a low rate of phagocytosis appeared when Solvent c was used.

In the electron microscopic study with serial, ultrathin sections, it was observed that pigment particles were surrounded by a limiting membrane.

Received 12/17/64. Accepted 10/ 3/66.