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The Distribution of Radioactivity within the Hepatic Cell after Administration of Labeled 3-Methylcholanthrene¹

Departments of Pharmacology and Anatomy, Baylor University College of Medicine, Houston, Texas 77025

Methylcholanthrene-¹⁴C or -³H was injected i.p. into rats. The maximal labeling in the liver occurred at 8–12 hr after administration of the isotope. The soluble cytoplasmic fraction contained over 80% of the liver radioactivity at all times. These chemical determinations were verified by autoradiographic studies.

The nature of the radioactivity in the liver was investigated by incubation of the soluble cytoplasmic fraction with RNase, DNase, and Pronase. Most of the radioactivity appeared to be protein-bound. The soluble fraction was also subjected to Sephadex G-200 chromatography. As early as 2 hr after administration of the isotope, the label appeared in high-molecular-weight material.

¹ These studies were supported by a grant from the American Cancer Society, E-373.

² Recipient of a Lederle Medical Faculty Award.

³ Predoctoral trainee of the USPHS.

Received 9/ 6/66. Accepted 10/24/66.

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