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Rates of Turnover of Deoxythymidine Kinase and of Its Template RNA in Regenerating and Control Liver¹

Department of Pharmacology, Baylor University College of Medicine, Houston, Texas 77025

Deoxythymidine kinase levels in liver were maximally enhanced by 12-fold at 24 hr after partial hepatectomy while aspartate transcarbamylase reached maximum activity by 48 hr. The rates of turnover of deoxythymidine kinase and of its template RNA were studied with the use of cycloheximide and actinomycin D, respectively.

The half-life (t) of the template RNA for deoxythymidine kinase in 24-hour regenerating and control adult liver was 7.5 and 3.0 hours, respectively. The t for aspartate transcarbamylase template RNA was >24 hours in both regenerating and control adult liver.

The rates of turnover of deoxythymidine kinase in regenerating and control livers were determined; the t was 3.7 and 26 hr, respectively.

It is concluded that immediately following partial hepatectomy, a substance is elaborated which may prolong the life of the deoxythymidine kinase messenger RNA by inactivating a catabolic mechanism and in this manner may ultimately lead to a greater intracellular concentration of enzyme.

¹ Supported by grants from the USPHS, National Cancer Institute (CA06571), and the National Science Foundation (GB4339).

Received 7/18/66. Accepted 10/ 7/66.