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Uptake of L-Histidine Alone and in the Presence of Other Amino Acids by Carcinogen-induced Sarcomas of the Rat in Vitro

Physiological Laboratory, University of Liverpool, Liverpool, England, and Department of Pathology, University of Sheffield, Sheffield, England

The uptake of free L-histidine by carcinogen-induced sarcomas and the effect of other amino acids on uptake have been investigated and compared with earlier work on a transplantable (RD3) sarcoma and on normal tissues.

The degree of uptake was considerably less than that found either with brain or with the transplantable sarcoma, and resembled more that found with intestinal mucosa, kidney, testis, and spleen. There was no relationship between degree of uptake and type of cell, weight of tumor, or age of tumor (as calculated from date of administration of carcinogen).

Neutral -amino acids produced considerable inhibition of uptake, with greater effect by the L isomer than by the D isomer; this was similar in general to the effect found with normal tissues, but different in detail. Long-chain basic -amino acids produced significant inhibition, but short-chain basic -amino acids produced none, a pattern found with none of the normal tissues investigated previously, and the opposite to that found with brain. Acidic -amino acids had no inhibitory effect, an effect opposite to that found with brain, but similar to that found in most cases with other normal tissues. -Amino acids had little or no inhibitory effect.

Received 7/22/66. Accepted 10/24/66.