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The Reduction of Folate and of Dihydrofolate by Homogenates of Leukocytes from Patients with Leukemia or with Myeloid Metaplasia¹

Laboratories of Pharmacology, Children's Cancer Research Foundation, and Departments of Pathology and Medicine, Harvard Medical School, Boston, Massachusetts 02115

Homogenates of human leukocytes reduced dihydrofolate more rapidly than folate. A correlation was observed between the assay of the enzyme with folate and with dihydrofolate. Homogenates of cells from patients receiving methotrexate reduced dihydrofolate much more rapidly than folate. Uninhibited enzyme was observed with both substrates in some homogenates of cells from patients receiving methotrexate. The level of enzyme activity in chronic myelogenous leukemia was correlated with the white blood cell count of the patient. Low levels of enzyme activity were observed in leukocytes from patients with myeloid metaplasia.

¹ This investigation was supported by Contract No. PH-43-62-169 from the Cancer Chemotherapy National Service Center of the National Cancer Institute and by Grant No. 6516 from the NIH.

Received 8/ 5/66. Accepted 1/ 9/67.