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Relationship of Infecting Dose to Recovery of Rauscher Murine Leukemia Virus (RMLV) in Random Bred Swiss Mice¹

Institute of Microbiology, Rutgers. The State University, New Brunswick, New Jersey 08903

Random bred Swiss mice were infected with graded decimal doses of Rauscher murine leukemia virus via the intravenous or intraperitoneal routes. Samples of spleen and plasma were then collected at various times after infection. Bioassay of these samples in recipient mice revealed a definite relationship between the amount of virus used to initiate the infection and the amount of virus recovered as measured by a spleen focus assay. Highest infective titers were obtained when the most concentrated inocula were used and the relative titers of virus in the pooled plasmas and spleens decreased as successively less concentrated inocula were used to initiate the infection. Plasma and spleen samples contained more virus when the inocula were injected intravenously than when injected intraperitoneally. In addition, virus was detectable earlier and persisted at higher levels when the initiating virus was injected intravenously rather than intraperitoneally.

¹ This investigation was supported by PHS Research Grant Nos. CA-04313-05 and CA-05175 from the National Cancer Institute.

² Present address: Department of Pathology, The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77025.

Received 12/12/66. Accepted 2/14/67.