This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Bonar, R. A. Articles by Beard, J. W. Search for Related Content PubMed PubMed Citation Articles by Bonar, R. A. Articles by Beard, J. W.

Ribonucleic Acid Components of BAI Strain A (Myeloblastosis) Avian Tumor Virus¹

Department of Surgery, Duke University Medical Center, Durham, North Carolina 27706

Sucrose-density-gradient centrifugation of RNA extracted from BAI strain A virus with phenol revealed 4 distinct components in continuous-flow spectrophotometer diagrams. Two of these, not previously described, sedimented at about 16 S and 26 S in association with the components of about 60 S and 5 S reported by others. The 5 S material, like cell transfer RNA, exhibited the amino-acid-acceptor activity already observed with whole virus RNA. Experiments indicated that the RNA's were not derived from external contaminating particulate or low molecular weight materials but that all were intrinsic to the agent. Comparative sedimentation studies showed that the 16 S and 26 S components were similar to cell ribosome RNA. Evidence indicated that the 16 S, 26 S, and a part of the 5 S materials were present in the cell cytoplasm and included in the virus during budding. The remainder of the 5 S appeared to be degradation products of the 60 S specific virus RNA component. The possible origin and biologic significance of the components are discussed, particularly with respect to mechanisms of virus formation.

¹ This work was aided by Research Grant C-4572 to Duke University from the National Cancer Institute and Training Grant 5 T1-GM-1019-04 from the National Institute of General Medical Sciences, NIH, USPHS; by a grant from the American Cancer Society, Inc. (E-84-A); and by the Dorothy Beard Research Fund.

² Studies in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Microbiology and Immunology in the Graduate School of Arts and Sciences of Duke University.

Received 10/13/66. Accepted 2/24/67.