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Metabolic Effects of Hydroxyurea on BHK-21 Cells Transformed with Polyoma Virus¹

Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032

Hydroxyurea interferes with the DNA metabolism of BHK-21 cells transformed by polyoma virus. The syntheses of RNA and proteins are not affected by concentrations of the drug which completely abolish DNA production. Evidence is presented which indicates that the metabolic block in DNA synthesis is not at the enzymic reduction of ribonucleotides to the corresponding deoxyribonucleotides. In BHK-21 cells, hydroxyurethan appears to have an effect similar to that of hydroxyurea. Hydroxylamine on the other hand was not found to be a specific inhibitor of DNA synthesis.

¹ This is the fourth paper in a series dealing with hydroxyurea. Paper III described the abnormal DNA isolated from hydroxyurea-treated bacteria (40). This investigation was aided by the Dr. George A. Carden, Jr., Special Fund for Cancer Research. Support was also provided by a USPHS Research Grant No. CA-08374 from the National Cancer Institute as well as by a contract [Nonr266(89)-NR 103–574] between the Office of Naval Research, Department of the Navy, and Columbia University.

² Research Career Development Awardee of the National Institute of General Medical Sciences, USPHS (5-K3-GM-29,024).

Received 10/20/66. Accepted 3/ 7/67.